Purpose: To find out how osmosis affects the cells of a carrot.
Hypothesis: If I place a carrot in water and another in a salt solution, then I will be able to see how osmosis affects the cells of a carrot.
Materials: 2 Carrots
2 (250 mL) Beakers
Procedures: 1.Fill two beakers half full with water.
2. Then, add two teaspoons of salt to one beaker.
3. After that, label the beaker with salt as “Salt Solution” and the beaker with water as “Water”.
4. Tie each carrot with a piece of string as tight as you can.
5. Now, place one carrot in the beaker labeled “Water”.
6. Place the other carrot in the beaker labeled “Salt Solution.
7. Next, wait 24 hours and then complete the observation chart in the observations section.
|Conditions after 24 hours||Water||Salt Solution|
|Thread was loose:|
|Thread was firm:|
|Decrease in cell size due to loss of water by the cell|
|Increase in cell size due to gain of water by the cell|
Conclusions: From this lab, I learned how osmosis, the diffusion of water molecules through a selectively permeable membrane, occurred with using the carrots, water, and salt water. It really showed how water molecules moved through the cell membrane by diffusing from an area where they are highly concentrated, to an area where they are less concentrated. For example, the water molecules in the carrot floating in the salt water solution diffused out into the salt water, causing the carrot to shrink and become limp. I saw how osmosis affects the cells of a carrot because the cells either diffused out causing the carrot to shrink or diffused into the carrot causing it to expand slightly and become rigid.
Answers to Questions:
Q #1: Which carrot demonstrated the process of turgor (rigidity)?
A: The carrot which was in the plain water demonstrated the process of turgor.
Q #2: Which carrot demonstrated the process of plasmolysis (shrinking)?
A: The carrot which was in the salt water demonstrated the process of plasmolysis.
Q #3: What kind of solution shows water loss?
A: That salt solution showed water loss.
Q #4: What might happen if red blood cells were used in the salt solution beaker?
A: Then the red blood cells would shrink as the result of the salt water solution.
Q #5: What might happen if we take the carrots we just used and placed them in reverse beakers overnight?
A: The one that was in the plain water before that was transferred into the salt water would shrink and the one that was in the salt water before that was transferred into the plain water would turn rigid and slightly bigger.